|
KMID : 0387720090200010046
|
|
Korean Journal of Blood Transfusion
2009 Volume.20 No. 1 p.46 ~ p.54
|
|
|
Development of Cryopreserved Red Blood Cell Panels for Verifying ABO and D Blood Grouping Reagents
|
|
Song Sung-Wook
Choi Jong-Hyeon
Kim Sin-Young
Kim Hyun-Ok
Min Hye-Kyoung
Kim Jae-Ok
Shin Won
|
|
|
Abstract
|
|
|
Background: ABO blood grouping reagent verification is essential to ascertain safe blood transfusions. However, the research use of donated blood products has been hampered in Korea by the blood transfusion law and management policies. In this study, we developed cryopreserved red blood cell (RBC) panels utilizing the high glycerol method to verify the ABO and D blood grouping reagents. In addition, we evaluated the stability of ABO and D antigenicity.
Methods: Fresh blood was frozen by the high glycerol method, aliquoted and cryopreserved in 2 mL cryotubes. Twenty-four vials of bloods with types A (n=5), B (n=5), AB (n=4) and O (n=10) for ABO RBC panels, and eleven vials of blood types D positive (n=5), D negative (n=5) and D weak (n=1) for D RBC panels were established. Potency, avidity and specificity tests were carried out with four different commercial ABO and D blood grouping reagents.
Results: The potency of cryopreserved RBCs after thawing showed no statistical difference compared with pre-freezing RBCs. Avidity time measurements were 5 seconds in ABO blood and 20 seconds in D positive blood. Specificity test uniformly showed 100% specificity. When thawed RBCs were stored at 4oC for 7 days, the potency test measured at intervals of 2 days showed no variation.
Conclusion: Cryopreserved RBC panels produced by the high glycerol method showed excellent results in stability test with reagents produced by manufacturers in Korea. Therefore, these panels can be utilized as a reliable method of verifying blood grouping reagents.
|
|
|
KEYWORD
|
|
|
Blood grouping reagent, High glycerol method, Cryopreserved red blood cell
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|